The cave of Agia Sofia is located 47km southwest of Chania, on the western walls of the gorge Topolia. The cave is located near the main road to Elafonisi. On the left of the cave entrance, there is the small church of Agia Sofia. The entrance of the cave has a width of 25m, while the height reaches 20m in many points. The cave has two rooms at different height, the surface of which is full of stalagmites.
In the cave, a clay figurine dating from the 4th century BC has been found. Moreover, Neolithic, Early Minoan, Late Minoan, Classical, Hellenistic and Roman pottery traces have been found.
The Cave of Agia Sofia is very important for biologists because it hosts the spider Pholcus creticus. The species is stenoendemic, i.e. has been identified only in this cave.